Wuhan Zeuschem Ltd.  

Product Name: Gastrin I Human Gastric Mucin 10047-33-3 with High Purity Factory Wholesale Model NO.: Gastric Mucin Purity: >99% State: Solid Suitable for: as Required Certification: GMP, ISO 9001, USP, BP Customized: Non-Customized Powder: Yes Other Packages: 1kg/Bag Terms: Fob, CIF Brand: Zeushcem Essay: UV, Htplc Price: Negotialble Trademark: zeuschem Transport Package: 25kg/Drum Specification: yellow powder Origin: China Product Description Chinese name: GastrinChinese synonym: mucin II; mucin III; gastrin; mucin, derived from porcine mucosal digestion; gastrin (pig); mucin (gastric mucin, mucin, gastrin); Gastrin / mucin II / mucin III / gastrin / MUCINEnglish name: Gastric MucinEnglish synonyms: Mucins, gastric; MUCIN FROM PORCINE STOMACH, TYPE III; mucin from porcine stomach; MUCIN, CRUDE, TYPEII; Mutin (gastric); Gastric Mucin; MUCIN; MUCIN GASTRICCAS number: 84082-64-4Molecular formula: NULLMolecular weight: 0EINECS number: 282-010-7Related Categories: Proteins; Peptides and Proteins; Biochemicals; Glycans; Carbohydrates; Enzymes; Organic Acids; Pharmaceutical Materials; Chemical Reagents; Additives; Biochemical Reagents-Proteins; Mucin; Proteins and Derivatives; Structural Proteins;-Mol File: Mol FileStorage conditions 2-8 ° CSolubility 1 M NaOH: soluble20mg / mLForm partially purified powderGastrin Usage And SynthesisChemical properties This product is white to yellow-white powder or yellow-brown granules, with peptone-like odor and salty taste. It has strong hygroscopicity and swells with water. The aqueous solution is gray or milky white. It does not precipitate when exposed to heat and precipitates when exposed to weak acids. It can be precipitated by more than 60% ethanol or acetone in solution. It can be decomposed into various proteins and polysaccharides when it is exposed to acid for a long time.Uses For the treatment of gastric, duodenal intestinal ulcers and hyperacidity.Uses For hyperacidity and gastric and duodenal ulcers.Production method Method one: ethanol precipitation methodThe gastric mucosa was minced, adjusted to pH 3 with hydrochloric acid, and digested at 40 ° C to obtain a digestive solution. At 0-5 ° C, ethanol was precipitated and filtered to obtain crude gastrin.Gastric mucosa → Gastric mucosa slurry [HCl] → [pH3, 40 ° C] digestive juice [ethanol] → [0-5 ° C] crude gastrinThe crude gastrin is degreased with ether, dried to obtain defatted gastrin, and pulverized to obtain a finished product.Crude gastrin [ether] → skim gastrin → finished productPreparation of Gastrin Capsules Gastrin capsules were prepared according to the requirements of capsules.Gastrin Products → Gastrin CapsulesMethod two: acetone fractionation methodThe gastric mucosa was minced into gastric mucosa slurry, adjusted to pH 2.5 with hydrochloric acid, and digested at 45 ° C. The digestion solution was further precipitated with cold acetone and filtered to obtain a crude gastrin.Gastric mucosa → Gastric mucosa slurry [HCl] → [pH 2.5, 45 ° C] digestive juice [cold acetone] crude gastrinAfter the crude gastrin is washed with ethanol, ether, defatted and dried, a gastrin fine product is obtained. The mother liquor is precipitated by cold acetone, filtered to obtain pepsin, and dried to obtain the finished pepsin product.Crude gastrin [ethanol, ether] → [washing, degreasing, drying] fine gastrinMother liquor [cold acetone] → [precipitated pepsin] pepsin [dry] → finished pepsin.Production method Method one, ethanol precipitation methodDigestion Mince the gastric serosa and place it in an acid-resistant sandwich pan. Under agitation, digest at a pH of 3-3.5, 40-45 ° C for 4h at a ratio of 1800ml HCl: 100kg gastric membrane, until the digestive juice is translucent. The double-layer gauze was filtered to remove impurities, and the pH was adjusted to 4-5 with 50% alkali to obtain digestive juice.Gastric mucosa [crushed] → gastric mucosal syrup [HCl, NaOH] → [pH3-3.5, 40-45 ºC] digestive juiceAlcohol precipitation Add more than 80% ethanol to the digestive juice while stirring, the alcohol content reaches about 70%, leave it at 0-5 ° C for 12 hours, collect the precipitate, and press dry. Add 60% -70% ethanol 2-3 times by wet weight, mix well, wash 1-2 times, press the pellet to dry, rub it, and dry it at about 70 ° C to obtain crude gastrin.Digestive fluid [ethanol] → [0-5 ºC, 12h] crude gastrinDegreasing and drying The crude gastrin powder was bagged, defatted with ether, and dried at 50 ° C with ether. Crush and pass through 80 mesh sieve to obtain gastrin.Crude gastrin [ether] → skim gastrin [crush] → finished gastrinMethod two: acetone fractionation methodDigestion Add 18ml HCl per 1000g of gastric mucosa, and stir at pH 2.5-3, 45 ºC for 3-4h to digest. The double-layer gauze was filtered to obtain digestive juice, which was stored in a cold storage below 5 ° C.Gastric Mucosal Serum [HCl] → [pH2.5-3, 45 ºC, 3-4h] digestive juiceThe gastrin was precipitated and the chemical solution was removed the next day. Stirring at room temperature and slowly adding acetone below 5 ° C so that the relative density of the solution reached 0.94-0.96. The gastrin was precipitated and filtered through double gauze to obtain coarse crystals. Washed twice with 70% ethanol. Dried at 70 ° C, degreased twice with ether, dried, and pulverized to obtain a fine gastrin.Precipitation of pepsin The mother liquor from which the gastrin was precipitated was stirred and supplemented with acetone below 5 ° C to make the relative density reach 0.89-0.91. It was allowed to stand for 4 hours to precipitate, and the supernatant was siphoned to recover acetone. The precipitate was collected, dried at 70 ° C, and pulverized to obtain a finished pepsin product.Mother liquor [cold acetone] → [relative density 0.89-0.91] pepsin [dry] → finished pepsin.